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After deparaffinization and hydration, the sections were treated with heated citrate buffer (pH 6.0), 0.3% H 2 O 2, and blocked with 3% BCA successively, then incubated with primary antibody at 4 C overnight and biotinylated secondary antibody

Tetracycline, specifically oral minocycline or doxycycline are most often used to treat granulomas due to their anti-inflammatory effect

Recent findings have even shown that functional abnormalities of the BBB represent additional pathological changes associated with MS (254)